GENDX NGS-TurboPrep for rapid, one-tube library preparation for HLA typing via Nanopore sequencing. Simplified workflow, no fragmentation, and ultra-fast processing.
GENDX NGS-Turbo enables high-resolution HLA typing from blood to results in just 3 hours. With rapid whole-gene amplification, a 30-minute library prep, and long-read sequencing on Oxford Nanopore Sequencing devices, it’s the ideal solution for time-sensitive transplant matching.
Human Leukocyte Antigen (HLA) typing to match patients and donors is an essential step taken to promote engraftment and reduce the risk of graft- versus host-disease.
Benefits
- Amplification & library preparation
- DNA to results ~2.5h
- 1 sample per run
Description
With a turnaround time of less than three hours (single sample) and an easy and robust workflow, NGS-Turbo® is designed for HLA typing in situations where time is a critical factor.
The NGS-TurboAmp kit offers 11 HLA loci using a whole-gene approach. The extremely quick 1-tube library preparation with NGS-TurboPrep requires no fragmentation and can be safely paused at any time. After nanopore sequencing the data can be directly analyzed with NGSengine®-Turbo.
Available as RUO.
Specifications:
- 1 sample per run
- Blood to results in 3 hours
- 11 loci
- No fragmentation, full phasing
- High gene coverage
- Long-read sequencing
- Short and simple workflow
- All incubations at room temperature
- Pause at any time
- Reuse flow cell multiple times
- Dedicated software NGSengine Turbo
- Low equipment costs
High-resolution HLA typing can be performed on multiple platforms, however, options are limited when time is of the essence. The MinION Mk1B and GridION sequencers from Oxford Nanopore Technologies are compatible with NGS-Turbo and provide long-read sequencing within a short timeframe, taking as little as 20 minutes for one sample.
With NGS-Turbo, the turnaround time for high-resolution HLA typing is only 3 hours, from blood to typing result.
Library preparation
With NGS-TurboPrep, library preparation can be performed in just 30 minutes. No fragmentation is required and intermission is possible at any time point.
Sequencing & data analysis
Finally, the sample can be sequenced on Oxford Nanopore Technologies sequencing devices and the data can be directly analyzed and typed with NGSengine-Turbo.
Compatibility
NGS-Turbo is compatible with R10.4.1 flow cells, and can be sequenced on MinION Mk1B and GridION sequencers, delivering rapid results in as little as 20 minutes per sample. The assay is to be used with compatible consumables from Oxford Nanopore Technologies, including SQK-LSK114 and EXP-AUX003.
NGS-Turbo has been validated to be compatible with MinKNOW versions 23.04.3, 23.11.7, and 24.06.5.