Quantabio sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing
Quantabio sparQ HiFi PCR Master Mix for high-fidelity, high-efficiency library amplification while maintaining even coverage
- HiFi DNA polymerase engineered to minimize amplification bias
- Increased amplification efficiency resulting in higher yields
- Uniform coverage across challenging AT- and GC-rich regions
- Robust amplification from input DNA as low as 250 pg
- Cost-effective alternative to KAPA HiFi with improved performance
sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The included primer mix allows amplification of DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina® sequencing platforms. The hot-start, proofreading DNA polymerase used in the sparQ HiFi PCR Mater Mix is specifically engineered to improve library amplification efficiency while reducing PCR-derived artifacts, resulting in higher library yields and better coverage uniformity. This kit supports low DNA input from 250 pg and efficient amplification of AT- and GC rich regions with minimal bias.