AccuStart II PCR SuperMix, 100 Reactions

Code: 95137-100

Quantabio AccuStart II PCR SuperMix is a robust, user-friendly 1-tube PCR SuperMix reagents for routine, general purpose PCR

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Quantabio AccuStart II PCR SuperMix is a robust, user-friendly 1-tube PCR SuperMix reagents for routine, general purpose PCR

  • User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and is impervious to repetitive freeze-thaw (≥ 20X)
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
  • Stringent, ultrapure antibody hotstart ensures sensitive and precise target amplification

AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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