PerfeCTa SYBR Green SuperMix, Low ROX, 2000 Reactions

Code: 95056-02K

Quantabio PerfeCTa SYBR Green SuperMix for sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry

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Quantabio PerfeCTa SYBR Green SuperMix for sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry

  • 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
  • Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
  • Supports efficient vortex mixing with proprietary anti-foaming formulation
  • SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)

PerfeCTa SYBR Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

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