Primary Cell Kit, P3, 4D-Nucleofector X Kit, 12 reactions, 100 µL Nucleocuvette Vessels

Code: V4XP-3012

For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format)

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The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.

  • High transfection efficiencies in primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector System
  • Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells
  • Low throughput applications

This kit has been determined to be optimal for transfecting the following cell types:

  • B cell (human)
  • CD34+ cell (human)
  • Chondrocyte (human)
  • DC (human)
  • DC (mouse), mature
  • ES (human), H9
  • ES (mouse)
  • Hepatocyte (human)
  • HMEC (human)
  • Keratinocyte (human)
  • Macrophage (human)
  • Monocyte (human)
  • Neuron (rat), brain Hi/Cx
  • NHBE (human)
  • RPE (human)
  • T cell (human), stim.
  • T cell (human), unstim.
  • T cell (mouse), unstim.

Includes:

  • 2 x 0.675 mL P3 Primary Cell Nucleofector Solution
  • 2 x 0.15 mL Supplement 1
  • 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)
  • 12 x 100 µL Nucleocuvette Vessels
  • 12 x Single use pipettes

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