Primary Cell Kit, P2, 4D-Nucleofector X Kit, 32 reactions, 16-well Nucleocuvette Strips

Code: V4XP-2032

For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-NucleofectorTM X Unit (20 µL format)

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The P2 Primary Cell 4D-Nucleofector X Kit S is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector System

This kit has been determined to be optimal for transfecting the following cell types:

  • Macrophage (mouse)
  • NHDF (human), neonatal

Applications

  • Transfection of low cell numbers in the range of 5 x 104to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Includes:

  • 1 x 0.675 mL P2 Primary Cell Nucleofector Solution
  • 1 x 0.15 mL Supplement 1
  • 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)
  • 2 x 16-well Nucleocuvette Strips (20 μl)