Cell Line Kit SE, 4D-Nucleofector X Kit, 32 reactions, 16-well Nucleocuvette Strips

Code: V4XC-1032

For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-NucleofectorTM X Unit (20 µL format)

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High transfection efficiencies in difficult-to-transfect cell lines. The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.

Applications

  • Transfection of low cell numbers in the range of 5 × 104to 1 × 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Includes:

  • 1 x 0.675 mL SE Cell Line Nucleofector Solution
  • 1 x 0.15 mL Supplement 1
  • 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0),
  • 2 x 16-well Nucleocuvette Strips (20 μL)

This kit has been determined to be optimal for transfecting following cell types:

  • 3T3-L1, diff., 3T3-L1, undiff.
  • A-10, A7r5
  • BHK-21, BJ
  • C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
  • DU145
  • GH3
  • HCT116, HeLa, HeLa S3
  • IMR-90
  • Jurkat (DSMZ), Jurkat E6.1
  • KG-1a, L6
  • MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
  • NCTC clone 929
  • Panc-1
  • T/G-HA VSMC, T-47D, T84
  • U2OS, U-87 MG
  • WI-38

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