Extraction Kit, E.Z.N.A. Endo-Free Plasmid DNA Mini Kit I, 50 preps

Code: D6948-01

Isolate low endotoxin (<1 EU/µg) plasmid DNA from up to 5 mL bacterial cultures using spin mini columns with E.Z.N.A. Endo-Free Plasmid Mini Kit I

Loading...


Add to Favourites
Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number. The E.Z.N.A.® Endo-Free Plasmid Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding conditions, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
  • Rapid – 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – Endotoxins efficiently reduced (<1 EU/µg)

Get our newsletter

Exciting news, new technologies, promotions, events, webinars and exclusive finds, straight to your inbox! Sign up for our newsletter.
We will protect your personal information. Read our privacy policy.