Tecan IBL International offers a comprehensive range of ELISAs, antibodies, immunoassays and recombinant proteins for research use only.
BoxA 2D: Mutant Form of BoxA for Negative Control
Product Overview: BoxA 2D is a mutant variant of BoxA, which is a DNA-binding domain of the HMGB1 protein. In BoxA 2D, the naturally occurring cysteine residues are replaced with aspartic acid. This protein variant, consisting of 89 amino acids, has a calculated molecular mass of approximately 10.4 kDa.
Key Characteristics of BoxA 2D:
- Function: BoxA 2D lacks the ability to inhibit HMGB1-induced migration of fibroblasts. This makes it an effective negative control for use in migration assays, allowing researchers to discern the specific effects of the wild-type BoxA protein.
- Purity: The protein is highly purified with trace amounts of LPS present (<0.4 ng/mg protein), minimizing potential contaminants that could affect experimental results.
- Usage: BoxA 2D is specifically designed as a negative control in experiments. It serves as a comparison against wild-type BoxA to evaluate the specific impact of BoxA on cellular processes and to confirm the specificity of BoxA’s activity.
Applications:
- Negative Control: Essential for experimental setups to distinguish between the specific effects of BoxA and background activity.
- Comparative Studies: Useful for comparing the functional effects of wild-type BoxA with its mutant form in various assays.
BoxA 2D is a valuable tool for researchers aiming to understand the specific actions of BoxA by providing a clear baseline in comparative studies.
This Immunoassay from Tecan IBL International is for research use only.
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