Amyloid-beta (x-40) ELISA

Amyloid-beta (1-40) ELISA Overview

Purpose: This ELISA assay is designed to measure Amyloid-beta (Aβ) peptides, specifically Aβ (1-40), which are cleaved at the N-terminal side. The assay focuses on quantifying Aβ (1-40) variants that may be present in biological samples.

Background:

• Amyloid-beta (Aβ): Aβ peptides are derived from the amyloid precursor protein (APP) through the actions of β-secretase and γ-secretase. The peptides can be 40 or 42 (or 43) amino acids long, with Aβ (1-40) being one of the primary forms.
• Plaque Formation: In Alzheimer's disease, plaques are primarily composed of Aβ peptides. These plaques are associated with neuronal damage and cognitive decline.

Significance:

• Alzheimer’s Disease: The accumulation of Aβ plaques, particularly Aβ (1-40), in the brain is a hallmark of Alzheimer's disease. The balance and distribution of different Aβ species are crucial in understanding disease pathology.
• Variant Detection: Research by Saido et al. in 1995 identified the presence of modified Aβ peptides, such as Aβ (N3pE), which are characterized by pyroglutamate formation from the glutamate residue at the 3rd position. This variant is significant due to its role in plaque formation and progression of Alzheimer's disease.

Assay Details:

• Measurement: This ELISA is designed to detect and quantify Aβ (1-40) in various samples. It can identify and measure the peptide’s levels and any associated variants.
• Application: The assay is used in research to study Aβ plaque formation, assess disease progression, and evaluate potential therapeutic strategies. It is applicable in experimental models and clinical research to understand the role of Aβ (1-40) in Alzheimer's disease and its impact on neurodegeneration.

Utility: By providing accurate measurements of Aβ (1-40) and its variants, this assay helps in elucidating the pathological mechanisms of Alzheimer’s disease and supports the development of targeted treatments.

This ELISA Kit from Tecan IBL International is for research use only.

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