Amyloid-beta (N3pE-42) ELISA

Amyloid-beta (N3pE-42) ELISA Overview

Purpose: This ELISA kit is designed to detect and quantify the human Amyloid-beta (N3pE-42) peptide. The assay specifically targets the pyroglutamylated form of Aβ, a key variant associated with Alzheimer’s disease.

Background:

• Amyloid-beta (Aβ) Peptides: Aβ peptides, derived from the amyloid precursor protein (APP), are central to Alzheimer's disease pathology. These peptides accumulate to form plaques in the brain, contributing to neuronal damage and cognitive decline.
• PyroGlu Variants: The pyroglutamylated form of Aβ, known as Aβ (N3pE), is produced through the conversion of glutamate to pyroglutamate at the 3rd position of the peptide. This modification stabilizes the peptide, making it resistant to further degradation by proteases and promoting its aggregation into oligomeric structures.

Significance:

• Plaque Composition: In Alzheimer's disease, plaques are predominantly composed of Aβ peptides, with Aβ (N3pE-42) being a significant variant found in the core of these plaques. The presence of pyroGlu variants is associated with enhanced plaque stability and a more aggressive form of the disease.
• Oligomer Formation: PyroGlu-modified Aβ peptides, such as Aβ (N3pE-42), rapidly form oligomers, which are thought to be particularly toxic to neurons. This makes the detection of Aβ (N3pE-42) crucial for understanding plaque formation and disease progression.

Assay Details:

• Measurement: This ELISA kit is tailored to detect human Aβ (N3pE-42) in biological samples, such as cerebrospinal fluid (CSF) or brain tissue extracts. It can provide accurate quantification of this specific Aβ variant, helping researchers investigate its role in Alzheimer's disease.
• Application: The assay is useful in research focused on Alzheimer’s disease, particularly in studies aiming to understand the role of pyroGlu-modified Aβ in plaque formation and neurodegeneration. It can also be valuable for evaluating potential therapeutic interventions targeting these specific peptide forms.

Utility:

• Disease Research: The ability to detect and quantify Aβ (N3pE-42) provides insights into the pathological mechanisms of Alzheimer's disease. This assay supports the development of targeted therapies aimed at modulating plaque formation and mitigating disease progression.
• Clinical Implications: Understanding the levels of Aβ (N3pE-42) could assist in assessing disease severity and response to treatment in clinical settings.

By focusing on the specific pyroGlu variant of Aβ, this ELISA kit offers a specialized tool for advancing Alzheimer's research and developing novel therapeutic strategies.

This ELISA Kit from Tecan IBL International is for research use only.

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